| Publication number |
CLPUB00800 |
|---|
| Authors |
Ali A.M., Ong B.K., Yusoff K., Hamid M., Ali S.A., Azimahtol H.L.P. |
|---|
| Title |
Generation of a stable hybridoma clone secreting monoclonal antibody against breast cancer. |
|---|
| Citation |
Asia-Pac. J. Mol. Biol. Biotechnol. 4:123-131(1996) |
|---|
| Abstract |
A stable hybridoma clone C2E7 was established as a result of fusion
between the lynphocytes of Balb/c mice sensitised with the MCF-7 breast
carcinoma cell line and Sp2/0-Ag14 myeloma cells. The clone showed 100%
positive after five limiting dilutions and secreted IgM monoclonal
antibody (Mab) with lambda, light chain. The Mab reacted strongly to breast
cancer lines MCF-7 and T47-D but showed neglible reactivity to other cell
lines. Immunohistological studies showed that the Mab reacted to lobular
breast and fibroadenoma cancer tissues at the cytoplasmic region. No
staining was observed when tested against normal mammary tissue or tissues
from uterine leiomyoma, stomach showing intestinal metaplasia, cervical
carcinoma, tonsilitis, neurofibroma and ductal pappiloma of the breast.
|
|---|
| Cell lines |
CVCL_E4IJ; C2E7 |
|---|
