| Publication number |
CLPUB00797 |
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| Authors |
Lallemand C., Tovey M.G. |
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| Title |
iLite reporter gene assay for quantification of the activity of anti-IL-23 and anti-IL-23 neutralizing antibodies. |
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| Citation |
(In conference) 11th annual Essential Protein Engineering Summit (PEGS Boston); pp.E135-E135; Cambridge HealthTech Institute; Boston; USA (2015) |
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| Web pages |
https://www.veritastk.co.jp/products/pdf/E-135-GB_Poster_IL-23_MT_PEGS2015_poster.pdf |
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| Abstract |
Interleukin 23 (IL-23) is a heterodimeric pro-inflammatory cytokine that
shares a common p40 subunit and a common receptor chain with IL-12. Both
cytokines exert however distinct non-redundant biological functions.
Conventional assays for IL-23 activity are based on the ability of IL-23
to support the proliferation of cell lines such as the IL-2 dependent
human T-cell line Kit 225, which has been reported to partially lose
dependence on IL-23, rendering the routine use of such assays problematic.
In order to reduce assay time from 2 days or more required for a
conventional IL-23 bioassay, to 6 hours or less, and to obviate non-
specific activation by other cytokines or growth factors with overlapping
biological activity, a reporter gene assay was established using the avian
B-cell line DT-40 that does not require IL-23 or other human cytokines in
order to proliferate and that is unresponsive to the growth factors
present in human serum.
|
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| Cell lines |
CVCL_E7RR;iLite IL-23 Assay Ready Cells |
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