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Cellosaurus publication CLPUB00774

Publication number CLPUB00774
Authors Sonobe H., Iwata J., Furihata M., Ido E., Ohtsuki Y.
Title Establishment and characterization of a new human malignant mesothelioma cell line, HS-Meso-1.
Citation Proc. Jpn. Cancer Assoc. 55:350.I340-350.I340(1996)
Abstract A number of established cell lines derived from human malignant mesothelioma has been reported. However, their morphological characteristics and tumor related oncogenes, have not fully investigated. We established a new cell line HS-Meso-1, from a tumor arising in the left anterior chest wall of a 70-year-old man. The cell line was analyzed cytogenetically, immunohistochemically, and genetically including p53, bcl-2 and mdm-2. The HS-Meso-1 cells in vitro were polygonal in shape, proliferating in a sheet-like pattern, and showed complex karyotypes with 45 to 99 chromosomes but no specific aberration. Heterografted nude mouse tumors revealed typical features of maligant mesothelioma, and were positive for vimentin, cytokeratin and p53 and bcl-2 proteins, but not for S-100 protein, EMA, CD68, or CD34. A point mutation of p53 gene from TAC to TGC at codon 236, as well as the increased m-RNA level of mdm-2, was detected with no rearrangement of bcl-2 gene. This cell line, exhibiting distinct morphologic features of malignant mesothelioma harboring a p53 point mutation, will be useful for biologic and pathologic investigations on this tumor.
Cell lines CVCL_E2T6; D-Meso-Sonobe