Abstract |
A number of established cell lines derived from human malignant
mesothelioma has been reported. However, their morphological
characteristics and tumor related oncogenes, have not fully investigated.
We established a new cell line HS-Meso-1, from a tumor arising in the
left anterior chest wall of a 70-year-old man. The cell line was analyzed
cytogenetically, immunohistochemically, and genetically including p53,
bcl-2 and mdm-2. The HS-Meso-1 cells in vitro were polygonal in shape,
proliferating in a sheet-like pattern, and showed complex karyotypes with
45 to 99 chromosomes but no specific aberration. Heterografted nude mouse
tumors revealed typical features of maligant mesothelioma, and were
positive for vimentin, cytokeratin and p53 and bcl-2 proteins, but not
for S-100 protein, EMA, CD68, or CD34. A point mutation of p53 gene from
TAC to TGC at codon 236, as well as the increased m-RNA level of mdm-2,
was detected with no rearrangement of bcl-2 gene. This cell line,
exhibiting distinct morphologic features of malignant mesothelioma
harboring a p53 point mutation, will be useful for biologic and
pathologic investigations on this tumor.
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