| Abstract |
A new cell-line, designated AMCC-1, was established from a mouse graft of
a human uterine cervical squamous cell carcinoma, and it has grown well
without interruption for over 30 months. The cultured cells of the AMCC-1
line were revealed spindle, polygonal and columnar in shape, with showing
a pavement-like arrangement and a tendency to piling up and lacking
contact inhibition. This cell line at passage 10 had a doubling time of
33.6 hrs, a saturation density of 5.98x10^4 cell/cm2, a plating efficiency
of 24.6%, and a mitotic coefficient of 5.4%. The chromosomal number of the
cell line varied widely and showed aneuploidy, while the modal chromosomal
number was stable in the diploid range. This cell line was able to
transplant into the subcutis of BALB/C nude mice and produced a non-
keratinizing squamous cell carcinoma resembling the original tumor. In an
ultrastructural examination of AMCC-1 cells, the squamous epithelial
origin of this cell line was confirmed. Also, the AMCC-1 cells at passage
10 produced squamous cell carcinoma related antigen (SCC-Ag) and
carcinoembryonic antigen (CEA), and released them into the culture medium.
At present, the AMCC-1 cells have undergone 66 passages. These cells have
maintained their tumorigenic capacity despite the prolonged maintenance
in culture and may be of utility in investigations of sensitivity to
radiation and chemotherapeutic agents, in studies of tumor associated
antigens.
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