| Abstract |
Cultivated cells as a substrate for virus replication are now an important
basis for a wide variety of experiments with viruses and for the problems
associated with them. Depending on the objective of the experiment and
other circumstances, either primary cultures are used for this purpose, i.
e. cultures established with cells freshly isolated from organs, or
cultures of passaged cell strains. As will be explained later, cell strain
cultures offer a number of fundamental advantages over freshly grown
cultures. However, our experience of handling cell strains, especially of
the changes that cultures can experience in the course of long-term
passages in vitro and their causes, is still incomplete. The following
description of a three-year-old cell strain from pig kidneys is therefore
intended as a contribution to clarifying these questions. Some authors
have already reported on attempts to keep cultures from pig kidneys in
continuous passages. However, details on the characteristics of their
strains or their behavior towards the foot-and-mouth disease virus have
not yet been published. Because of the practical importance of cultivating
this virus in kidney cell cultures, the behavior of the cell strain
obtained in relation to the FMDV virus is discussed in detail.
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