| Abstract |
Fc gamma receptor 3B (FcgammaRIIIB, encoded by the gene FCGR3B) plays a
crucial role in immunity response activated by cellular effectors and
regulatory functions. Copy number variation (CNV) of this gene has been
previously reported to affect susceptibility to several diseases such as
autoimmune diseases and chronic inflammatory response. One of the rarest
and smallest tribes of Orang Asli which is Negrito-Mendriq sample was used
as a subject in this study. Twenty-two (22) lymphoblastoid cell lines
(LCLs) of Negrito-Mendriq was obtained from the deposited cell archive,
Institute Medical Molecular Biotechnology, IMMB (UiTM Sg Buloh, Selangor,
Malaysia) and the LCLs were revived. The DNA was extracted from the LCL
and finally PRT - REDVR assay was carried for FCGR3B copy number
genotyping. Analysis revealed that copy number 2 demonstrated the highest
distribution for FCGR3B (90.9%); whilst no copy number loss was found for
FCGR3B. For high copy number (>2), 9.1% was obtained. Copy number greater
than 3 (>3) was not found in this study. Finally, it was concluded that no
significant difference of CNV of FCGR3B for Orang Asli Negrito-Mendriq
when compared with the reported studies. CNV of FCGR3B genes of Negrito-
Mendriq was found normal in this study, where the pattern of copy number
distribution was almost similar with previously reported studies.
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