| Publication number |
CLPUB00733 |
|---|
| Authors |
Katsuaki Matsui, Tokichi Yumoto, Junko Nashiro, Kimio Hashimoto, Kiyoo Furuse, Kanehito Morimoto, Masahiro Akeho, Iwao Maeyama; |
|---|
| Title |
Establishment of a cell line derived from human osteosarcoma. |
|---|
| Citation |
Ryukyu Daigaku Hoken Gaku Igaku Zasshi 5:187-195(1982) |
|---|
| Web pages |
https://hdl.handle.net/20.500.12000/0002016471 |
|---|
| Abstract |
Establishment of the cell line (HOS-O) of osteosarcoma was successfully
accomplished. The materials for this study were obtained from surgically
removed tumor mass metastasized to the lung. The patient was a 10 year-old
girl, suffering from an original tumor in the right distal femur. The
tissue culture has been started in September 1977, and the cell line has
been maintained in a good condition for about five years. The shape of
cultured cells is elongated-spindle and its chromosome number is 46. By
histochemical examination, the cells revealed weakly positive at alkaline
phosphatase, and moderately positive at acid phosphatase, and highly
positive at nonspecific esterase and 5'-nucleotidase. Each of twice
transplantations of those cells, to the back of nude mice, was successful.
Histological features of the trasplanted tumors were resembled to that of
the original sarcoma, showing a remarkable formation of osteoid tissue.
|
|---|
| Cell lines |
CVCL_C7SI; HOS-O |
|---|
