| Publication number |
CLPUB00721 |
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| Authors |
Burand J.P., Harrison R.L., Boucher M. |
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| Title |
Establishment of a winter moth, Operophtera brumata, cell line permissive for OpbrNPV replication. |
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| Citation |
(In) International congress on invertebrate pathology and microbial control and the 48th annual meeting of the Society for Invertebrate Pathology; pp.99-99; Vancouver (2015) |
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| Web pages |
https://docplayer.net/123138184-International-congress-on-invertebrate-pathology-and-microbial-control-and-the-48th-annual-meeting-of-the-society-for-invertebrate-pathology.html |
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| Abstract |
The winter moth, Operophtera brumata, is an invasive lepidopteran pest on
deciduous tree species in the Northeast United States and Canada. We have
recently found the previously identified nucleopolyhedrovirus OpbrNPV in
O.brumata in Massachusetts and, as an initial step in developing this virus
for the control of winter moth, we have established a cell line from this
insect. Embryonated winter moth eggs were dissected, and pieces of embryo
were transferred to TNM-FH and ExCell420 media supplemented with fetal
bovine serum to establish primary cell cultures. Cultures were incubated
at 19 Celsius and sub-cultured using trypsin to produce an embryonic cell
line, designated IIBBL-ObE1. The initial infection of this cell line was
accomplished at passage 15 using occlusion-derived virus, and appearance
of occlusion bodies (OBs) inside the nuclei of infected cells occurred
with one week post infection. OBs recovered from infected cells were
confirmed to be OpbrNPV by PCR and were infectious when fed to winter moth
larvae.
|
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| Cell lines |
CVCL_C2QK; IIBBL-ObE1 |
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