| Publication number |
CLPUB00718 |
|---|
| Authors |
von Bulow M., Scharfe T., Kloppel G., Kern H.F. |
|---|
| Title |
Establishment and characterization of continuous tumor-cell lines from human pancreatic carcinoma in vitro. |
|---|
| Citation |
Digestion 25:17-18(1982) |
|---|
| Web pages |
https://www.karger.com/Article/Abstract/198813 |
|---|
| Abstract |
Nine different cell lines from human exocrine pancreatic cancer were
established in tissue culture. Five of these derived from human cancer
tissue transplanted on NMRI nu nu mice. 4 were established from primary
tumors or metastatic lymph nodes recovered during surgery. The tumor
tissue was minced and trvpsinised to obtain a single cell suspension. The
cells were grown in DMEM with 10% FCS and antibiotics. Each cell line
showed an individual morphological pattern of growth which remained
constant throughout all passages. The cells generally showed good
adherance to plastic with a variable portion of cells growing in
suspension. These 'floaters' seem to he characteristic for pancreatic
cancer grown in tissue culture. We found very good correlation between the
histological grade of the primary tumor and growth kinetics of the
respective cell line in vitro. Lines derived from low grade tumors were
slow-growing with long replication times in culture as well as in the nude
mouse. Cell lines from grade III tumors on the other hand would divide
rapidly in vitro with a very high percentage of nonadherent floaters,
showing a multicolony pattern of growth. After an average of 5 passages in
vitro. the lines were passed back into nude mice. where they promptly
produced tumors of histomorphology identical to that of the primary
carcinoma. Cytological studies as well as electron microscopy proved the
cultured cells to be morphologically identical to those from the primary
tumor. We established a clonogenic semisolid agar assay for chemotherapy
studies. The cell lines have so far not shown pronounced sensitivity to
any of the comercially available drugs. With the establishment of
continuous in vitro lines from human pancreatic carcinoma. we have a
powerful tool to study the biology of this so far uncurable tumor. They
are of great value for in vitro sensitivity testing with cytostatic
agents, as well as in the production of monoclonal antibodies for
immunodiagnosis or therapy of this tumor.
|
|---|
| Cell lines |
CVCL_C1YF; PC-2 [Human pancreatic carcinoma Germany] CVCL_4001; PaCa-3 CVCL_7087; PaCa-44 |
|---|
