| Publication number |
CLPUB00689 |
|---|
| Authors |
Lukash L.L., Iatsyshyna A.P., Pidpala O.V., Vagina I.M., Kochubey T.P. |
|---|
| Title |
Establishment of new lines of murine stem cells and investigation of the influence of microenvironment on their karyotypical variability in vitro. |
|---|
| Citation |
Fiziol. Biokhim. Kul't. Rast. 38:144-152(2006) |
|---|
| Web pages |
https://eurekamag.com/research/012/970/012970119.php |
|---|
| Abstract |
New lines of murine stem cells G1, G4, G6 and G7 have been obtained. They
have been passed in vitro during from 50 to 140 passages without
appearence of crisis in cellular populations. When cultivating in
monolayer the cells had similar fibroblast-like morphology. The
cytogenetical investigation has been carried out after long time cell
growth at the conditions established for standard monolayer cultures and
at the conditions developed for stem cells. Comparative cytogenetical
analysis of cell samples isolated from populations of G1, G4 and G7 have
revealed manifestation of genomic and chromosomal instability after long
time cultivation and dependence of karyotype changing on the conditions of
microenvironment.
|
|---|
| Cell lines |
CVCL_B6W8; G1 [Mouse germ stem cell] CVCL_B6XA; G4 [Mouse germ stem cell] CVCL_B6XB; G6 CVCL_B6XC; G7 |
|---|
