| Abstract |
The work of culturing hemocytes from larvae of Mythimna separata Walker was
published in 1980. By using culture medium BML-TC 10 supplemented with
calf serum and Hepes, cells can be transferred successfully and thus a new
cell line is established. Up to now it has been transferred more than 100
passages, Four cell types - plasmatocyte, prohemocyte, granulocyte and
spherulocyte are found in our culture, within which plasmatocyte seems to
be the most prominent cell type, and the leaf-like and vermiform
plasmatocytes are all found. The cells are mainly suspended in culture,
only a few cells occasionary attach to bottle loosely. But when the cells
in 16th passage are recovered from liquid nitrogen and cultured in BML-TC-10
medium, whether Hepes is added or not, the cells attach to bottle
firmly, many of which arc spindle in shape; and at the beginning of this
recovery experiment, if the medium BML-TC 10 is supplemented with
yeastolate, the cells become suspending just the same as original culture.
But later, although yeastolate is added, the cells still remain the
attached type. Hepes, as an buffer agent, has been reported to have the
ability to support the long-term division potential in tissue culture
system. But recently, authors notice that it is injurious to cells and
suggest it be used carefully in tissue culture. In our culture the cell
line has been established after the using of Hepes, no side-effect has
been observed obviously until now, whether there is any ill effect of
Hepes upon the cells will be studied further. The work of establishment of
cell line from hemocytes of Mythimna separata was not known before, it is
the first time to establish this cell line, and the experiment of
infection of NPV upon the cells will be going on.
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