| Publication number |
CLPUB00643 |
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| Authors |
Shi C.-H., Zhang H., Xi L., Wang X.-W., Wang L.-M., Zhao Y. |
|---|
| Title |
Establishment of a stable cell line expressing the fusion protein of Mycobacterium tuberculosis Hsp65 and IL-2. |
|---|
| Citation |
Zhongguo Ren Shou Gong Huan Bing Xue Bao 24:718-721(2008) |
|---|
| Web pages |
http://www.rsghb.cn/EN/Y2008/V24/I08/718 |
|---|
| Abstract |
The genes encoding Mycobacterium tuberculosis Hsp65 and IL-2 were cloned
into eukaryotic expression vector pcDNA3.1 and transformed to E.coli DH5a.
Then, the positive recombinant plasmid Hsp65-IL-2-pcDNA 3.1 was selected
and transfected to P815 cells (H-2 d) whose genetic background was identical
to that of BALB/c mice. In this way, one positive cell clone was selected by
G418, and the specific mRNA of the fusion protein was detected by RT-PCR. By
immunofluorescence technique (IFT) the P815 cells transfected with
recombinant plasmid showed strong fluorescence, indicating the expression
of fusion protein in this cell. It is apparent that a stable cell line
expressing M.tuberculosis Hsp65 and IL-2 proteins in P915 cells is
obtained, which may be used as the optimal target cell for CTL experiment.
|
|---|
| Cell lines |
CVCL_A9JC; P815 Hsp65-IL-2 |
|---|
