Cellosaurus logo
expasy logo

Cellosaurus publication CLPUB00642

Publication number CLPUB00642
Authors Chen N.-H., Wang X.-W., Li Y.-F., Jiang P.
Title Cloning and identification of PK15 cells for the sensitive replication of porcine circovirus 2.
Citation Zhongguo Shou Yi Ke Xue 41:14-18(2011)
Web pages
Abstract To improve the titers of porcine circovirus 2 (PCV2), PK15 cells were cloned using the limiting dilution method and the susceptible cells to PCV2 were selected by indirect immunofluorescence assay. In result, one clone, PK15-B1, could reliably produce PCV2 with high titers was selected from heterogeneous PK15 parental cells. The viral titer could be as high as 107.0TCID50/mL after the PK15-B1 cells inoculated with 100 TCID50 for 3 days. The levels of the viral titer and growth speed in PK15-B1 cells were significantly higher than those in PK15 parental cells. Moreover,the clone cells did not contaminated with porcine circovirus 1, bovine viral diarrhea virus, classical swine fever virus, encephalomyocarditis virus or mycoplasma. The results indicated that the PK15-B1 cell clone is reliably permissive to PCV2 replication and could be used to study the biological characterization of PCV2 and replication of PCV2 in vitro.
Cell lines CVCL_A9EE; PK15-B1