| Abstract |
By using the technique of tissue culture, we successfully obtained the
cell from goldfish fin, skin, eye, heart, spleen, spermary, swim bladder
and kidney. Among them, GFF (cell from goldfish fin) has the strongest
proliferating ability. Tests of high times dilution, decreasing the
percent of newborn calf serum and freezing conservation with GFF indicate
that high times dilution cell still have the normal proliferating ability;
GFF can be cultured in H-MEM-5; after defrosting, GFF still maintained
high proliferating ability and the cell survival rate is 85%. At the same
time, GFF was inoculated with herpes virus of goldfish, after CPE
appeared, the passaged virus was observed by electron microscope. The
results indicate that GFF cell can sustain the propagation of herpes virus
of goldfish, and this established the basis of researches about goldfish
virus.
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