| Abstract |
The first successful propagation of Cowdria ruminantium in vitro was
reported by Bezuidenhout et al in 1985, who infected a cell line, E5,
derived from bovine umbilical endothelium. Prior to this, all cell culture
systems tested allowed little more than limited maintenance of infectivity
of the organism (reviewed by Uilenberg 1983; Bezuidenhout et al 1985; and
Bezuldenhout 1987). Recently, a primary neutrophil culture system for
Cowdria was devised by Logan et al (1987) which, while also of short
duration, did permit intracellular multiplication of the rickettsia with
production of antigen in amounts adequate for serological tests. In this
paper, we report results of efforts to cultivate the heartwater organism
in 11 cell lines other than the E5 line used by Bezuidenhout et al (1985)
and Bezuidenhout (1987).
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