| Abstract |
Hybrid culture A4xC obtained through cell fusion of SPEV-TK-and pig
splenocytes is capable of virus induced interferon formation. Epithelium-
like cells are prevalent in the cell culture (60%) and lymphocyte-like
cells of round shape with dense nucleus and thin rim of the cytoplasm
account for 40% and more. We have determined that the culture keeps its
cultural and morphological characteristics within 98 passages and afer
long maintenance in liquid nitrogen. The 97-th passage showed 2 peaks of
modal class chromosomes 36 and 38 in 64-56% of the cells respectively.
Proliferation indey is 4.45-5.14. 24 hours after infection of the culture
by production "K" strain of the European Swine Fever Pestivirus we could
observe gemmation of the cytoplasm in the form of a "crown" as well as
nuclei pyknosis, condensation of chromatin blocks near the nucleus
membrane and nucleolus hypertrophy. Later, on the 4-th day degenerative
changes in the monolayer were observed. 8 hours after infection by the
virus of pig encephalomyocarditis degeneration of cells starts,
cytoplasmatic inclusions appear and cytoplasm vacuolization, nuclei
pyknosis and pathological mitosis take place. Monolayer is completely
destroyed in 17 hours. After infection of the culture by equine herpes
virus (1st type of the strain SV/69) the cells became rounded, multinuclei
symplasts were formed, nuclei deformed and pyknotied and intranuclear
infusion could be observed. On the 3rd day all the cells perish. The hybrid
cell culture A4xC has stable cultural, and morphological characteristics,
it is sensitive to the viruses of European Swine Fever Pestivirus, pig
encephalomyocarditis, equine rhinopneumonia. It is recommended for use in
virology and biotechnology.
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