| Abstract |
We established a novel megakaryoblastic leukemia cell line (TS9;22) and
assessed its characteristics. TS9;22 cells carry the Ph translocation and
major-BCR rearrangement shown by Southern analysis to be those of the
original patient's sample, therefore, we identified TS9;22 cell as a
leukemic cell line. TS9;22 cells carry both myeloid (CD 13 and CD33) and
megakaryocyte/platelet antigens(CD41). Addition of interleukin(IL)?3 and
IL-6 caused increasing numbers of colony formation and proliferation, as
well as increasing number of polyploid cells with up to 12 nuclei with
mature megakaryocyte morphology. Moreover, IL-3 and IL-6 induced
expression of megakaryocyte/platelet antigen as well as demarcation
membrane and platelet-associated granule-like structure. TS9;22 also
constitutionally expressed transcription factors, GATA-1/GATA-2/SCL
expression, that were considered to be associated with
erythoid/megakaryocyte lineages. All these findings indicate that TS9;22
could be a useful cell line in determining megakaryocyte lineage
differentiation.
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