| Abstract |
In this paper, the chromosome aberration of long-term cryopreserved and
subcultured Francois' monkey (Semnopithecus francoisi) cell line (KCB
92008) was analyzed by fluoresence in situ hybridizaton (FISH) using human
9 and 14 chromosome DNA probes. After comparing the hybridization pattern
with the G-banding pattern on the same metaphase, a translocation between
Nos 12 and 17 chromosomes was identified in some Francois' monkey cells,
one of chromosome No 17 was broken into two at the breakpoint 17q13, the
segment (17q13-17qter) without centromere transfered to the long arm
terminal of one chromosome No 12. Thus, two derivant chromosomes der(12)
and der(17) were formed, the long arm of der(12) was longer than the
normal partner, while the long arm of der(17) was shorter than the normal
one. The result indicated that the technique of FISH using human whole
chromosome probes was not only a powerful tool to detect human chromosome
rearrangements, but also a useful method to study the primate chromosome
aberration.
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