| Publication number |
CLPUB00572 |
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| Authors |
Fayet G., Hovsepian S. |
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| Title |
Strategy of thyroid cell culture in defined media and the isolation of the OVNIS and PORTHOS cell strains. |
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| Citation |
(In) Progress in endocrine research and therapy, Vol. 2, Thyroglobulin -- the prothyroid hormone; Eggo M.C., Burrow G.N. (eds.); pp.211-224; Raven Press; New York (1985) |
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| Abstract |
In this paper, we describe some characteristics of two ovine and one
porcine thyroid cell strains. The porcine cells grow in monolayer in 6H
plus 0.1 % C.S. enriched synthetic medium. The ovine cells are grown
routinely in either 6H or 5H (without TSH) media containing 0.1 % C.S. The
three cell strains are two year-old. All are sensitive to insulin,
thyrotropin and hydrocortisone. The other three "hormones" do not modify
growth. However the combination of somatostatin and glycyl-histidyl-lysine
is slightly inhibitory for growth. In contrast with the FRTL line, the 5H
ovine cells are not TSH-dependent for growth. Furthermore, the 6H cells
grow in absence of serum, synthesize thyroglobulin at high level even in
the last passages tested and formed follicles during one year and a half
despite subculturing. The cell strains were named PORTHOS (Porcine Thyroid
"0" Serum) and OVNIS (Ovine "Nihil" Serum) and represent new tools in
thyroid cell studies.
|
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| Cell lines |
CVCL_A9I7; OVNIS 5H CVCL_3409; OVNIS 6H CVCL_2695; Porthos |
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