| Abstract |
Attempts were made to establish new cell lines from the embryos of
Helicoverpa armigera (Hubner) to study its susceptibility to baculoviruses.
Three primary cell cultures were initiated from the embryo of H. armigera.
Among the three cultures, HaE9 alone produced sufficient cell growth to
allow subculturing and led to establishment. Population doubling time
(PDT) of the cell line HaE9 ranged from 3.63 days to 4.75 days for five
consecutive generations. Susceptibility studies of different baculoviruses
revealed that HaE9 was permissive for HearNPV alone. Studies on the
influence of incubation temperature regimes (26, 27 and 28 Celsius) on cell
infection and polyhedral occlusion body (POB) yield of HearNPV in HaE9
cell line revealed that the temperature regime of 27 Celsius could yield a
maximum of 5.63 and 5.75x10^7 POB/ml in 20th and 21st passage respectively.
Bioassays with cell cultured viruses in comparison with in vivo produced
virus had similar effects against 2nd instar H. armigera larvae with LC50
values of 0.029 and 0.017 POB/mm2, respectively.
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