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Cellosaurus publication CLPUB00532

Publication number CLPUB00532
Authors Koike M., Sato K.
Title Culture of insect cell lines originated from Mamestra brassicae with autoclaved serum-free medium.
Citation (In) Invertebrate and fish tissue culture; Kuroda Y., Kurstak E., Maramorosch K. (eds.); pp.7-8; Japan Sci. Soc.; Tokyo (1988)
Web pages https://www.springer.com/de/book/9783642736285
Abstract Some cell lines originated from M.brassicae are able to be subcultured with an autoclaved serum-free medium. The medium was modified to omit components of sodium hydrogen carbonate, sodium dihydrogen phosphate and serum. These cell lines were SES-MaBr-1, -2, -3, -4 (from fat cells), NIAS-MaBr-92, -93 (from hemolymph), NIAS-MaBr-32 (from ovary). An inoculum of 2x10^5 cells/ml of each cell line produced more than a concentration of 10^6 cells/ml in stationary cultures (5ml). In a spinner flask culture of 500ml, MaBr-93 grew to 1x10^6 cells/ml from 2x10^5 cells/ml. In a jar fermenter culture of 40 liters, it grew to 6.5x10^5 cells/ml from 1.5x10^5 cells/ml.
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