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Cellosaurus publication CLPUB00527

Publication number CLPUB00527
Authors Manaka K.-I., Nonaka N., Yamada T., Hirai H.
Title Monoclonal antibodies against large-cell carcinoma of the lung.
Citation Dokkyo J. Med. Sci. 12:31-41(1985)
Web pages https://dl.ndl.go.jp/info:ndljp/pid/11023962
Abstract Monoclonal antibodies (MoAbs) against a newly established cell line (HLC-2) derived from a human large-cell lung carcinoma were prepared by hybridization between the murine myeloma P3U1 and spleens from immune BALB/c mice. The specificity of the MoAbs was screened with the panels of non-neoplastic or neoplastic cultured cells and the tissues of the same cancer patient as immunogen donor by using antibody-dilution curves and histological preparations of avidin-biotin-conjugated-peroxidase antibody technique. Several MoAbs reacted against immunogen cultured cells (HLC-2) and autologous tumor tissues. Among those MoAbs, two MoAbs (1A7 and 6B3) reacted at very low or undetectable levels with non-neoplastic cells (primary-cultured lung epithelial cells and the autologous lung tissues). In the lung carcinomas, MoAb 1A7 did not react against QG-90 cells (small- cell carcinoma), PC-13 (large-cell carcinoma) and SK-MES-1 cells (squamous cell carcinoma), but reacted against Luci 10 cells (adenocarcinoma). And MoAb 6B3 reacted only against SK-MES-1 cells and HLC-2 cells. Both MoAbs did not react against other types of tumor cell line (HeLa, uterus carcinoma and HuL-1, hepatoma). That is, the epitope recognized by MoAb 1A7 exists possibly associating with adenocarcinoma and normal glandular epithelium of the lung. That of MoAb 6B3 is suggested to associate with squamous-cell carcinoma and large-cell carcinoma and to be specific in neoplastic cells of the lung. The monoclonal antibody-dependent macrophage- mediated cytotoxicity assay was performed. The inhibition activities of MoAbs to the target cell growth were found to be enhanced by mixed cultures with macrophages. And MoAbs 1A7 and 6B3 showed a strong inhibitory effect. All these findings suggest the potential of the identification of the molecules of lung cancer-associated antigens and the application on the clinics.
Cell lines CVCL_WZ40; 6B3 [Mouse hybridoma against human LGALS3BP]