Cellosaurus publication CLPUB00512

Publication number	CLPUB00512
Authors	Enrique I. Miranda, Juan Jose Montesinos, E.E. Lopez, Adolfo Martinez, Juan Collazo-Jaloma, P. Perez, Hector Mayani, Alejandro Manuel Garcia-Carranca, Alejandro Zentella-Dehesa, Mario Gutierrez;
Title	Establishment and characterization of a Hodgkin's lymphoma cell line.
Citation	Blood 94 Suppl. 1:250b.4323-250b.4323(1999)
Abstract	In order to gain more knowledge in the biology of Hodgkin's lymphoma, we have established a new cell line (EuHe) from a 15 year-old girl with mixed cellularity Hodgkin's disease. A lymph node biopsy of patient was explanted and kept in culture using conventional tissue culture methods. Cytogenetic methods were used for karyotype analysis. Clonogenic assay was applied to test cloning efficiency. Flow cytometry was used for cell cycle analysis. The tumorigenic capacity of the cells was determined by inoculating them into athymic nude mice. Immunological and cytochemical staining were performed using conventional techniques. The EuHe cells are CD20 antigen positive and their population doubling lime is 16 hrs. In addition, the EuHe cells possess a complex karyotype with several abnormalities. Cloning efficiency in soft agar was found to be >60%. They produced tumors when 10^6 cells were injected into NIH Swiss and NCr strains of athymic nude mice. The figure shows a cytospin preparation of EuHe cells stained with Wright. The EuHe cell line is suitable for studying the nature of B lymphomas and testing compounds against lymphoproliferative disorders.
Cell lines	CVCL_WT21; EU-HE