| Publication number |
CLPUB00511 |
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| Authors |
Bierkens J.G.E.A., Maes J., Ooms D., Collier M., Vangenechten C., Borremans B., van Vlasselaer P., Schoeters G. |
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| Title |
Decreased acquisition of osteoblastic phenotype markers and increased response to interleukin-1 and parathyroid hormone in pre-osteoblast like cells under microgravity. |
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| Citation |
(In book chapter) Life sciences research in space. Proceedings of the fifth European symposium held 26 September-1 October 1993 in Arcachon France, ESA SP-366; Oser H., Guyenne T.D. (eds.); pp.25-30; European Space Agency Publications Division; Noordwijk; Netherlands (1994) |
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| Web pages |
https://articles.adsabs.harvard.edu/full/1994ESASP.366...25B |
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| Abstract |
Some evidence exists that the response of bone to microgravity is
osteoblast mediated. In order to test this hypothesis we have made a
comparative study in space and on earth of the acquisition of early
osteoblastic phenotype markers (ALP and Collagen type I) in a pre-
osteoblastoid cell line MN7 mounted on a collagen matrix. In addition, the
response of MN7 cells to two potent bone effectors. 11-1 (1 ng/ml) and FTH
(10-7 M), was studied under micro- and unit gravity. The results show
that although the proliferation of the MN7 cells is not affected by
microgravity, differentiation as measured by the expression of ALP and
collagep type I, is slowed down In contrast. interleukin-1 and PTH seem
to elicit a larger response in space than on earth. These results may
indicate that microgravity alters bone metabolism in space by a dual mode
of action: (i) suppressed differentiation of pre-osteoblasts towards
mature osteoblasts and (ii) an exaggerated response to bone resorption
inducing effector molecules in osteoblasts.
|
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| Cell lines |
CVCL_WS34; MN7 |
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