| Abstract |
Monoclonal antibodies (TH 69, TH 126, TC 25) were raised by immunization
of mice with T-cell acute lymphoblastic leukemia (ALL) cell lines CEM and
HSB-2. Analyzing blood cells from normal donors (n = 10), TH 69 and TH 126
did react with approx. 30% of T-cells, independently whether of helper or
suppressor/cytotoxic phenotype, while the determinant detected by TC 25
was expressed only on platelets and, less intense, on monocytes. A variety
of human cell lines (n > 30) was employed for characterizing antibody
specificity. All three antibodies did react with 4 of 4 T-ALL lines, but
little reactivity was seen outside the T-lineage. Fresh Sezary cells as
well as Sezary line HUT 78 were stained only by TH 126. Testing leukemias
and lymphomas (n > 25), only TC 25 showed major reactivity outside the
T-cell tumors, namely on chronic myelogeneous leukemia in blast crisis and
very immature acute leukemias. Thus, while TH 69 and TH 126 will help to
dissect T-cell tumors, TC 25 may be useful in analysis of CML in blast
crisis and immature leukemias.
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