| Publication number |
CLPUB00500 |
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| Authors |
Collins J.K., O'Mahony A., O'Brien F., Corbett A., Morrissey D., O'Donoghue M., O'Sullivan G.C. |
|---|
| Title |
Evaluation of newly established cell lines as models to study growth, invasion and metastatic spread in oesophageal cancer. |
|---|
| Citation |
Fibrinolysis 6 Suppl. 4:83-88(1992) |
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| Web pages |
https://eurekamag.com/research/007/320/007320341.php |
|---|
| Abstract |
Three oesophageal cell lines were established from two squamous carcinomas
and one from an adenocarcinoma. Each cell line expressed both cytokeratin
and vimentin intermediate filaments. They exhibited clonogenic growth in
serum free media and grew in an anchorage independent fashion in soft agar.
Each cell line produced both mitogenic and transforming growth factor
activities. All three cell lines were invasive in the in vitro chick heart
fragment invasion assay, albeit to different degrees, with OC2 cells
showing the highest degree of invasiveness. In tumourigenicity studies in
athymic nude mice, all three cell lines induced primary subcutaneous
tumours at the site of injection. OC1 and OC3 cells gave rise to
metastases, while the OC2 cells, although the most rapidly growing, failed
in every case to metastasize. OC1 and OC3 cells were found to express
urokinase-type plasminogen activator (uPA), while OC2 cells did not. Our
findings suggest a role for uPA in conferring metastatic potential on
oesophageal carcinoma cells.
|
|---|
| Cell lines |
CVCL_WL07; OC-1 CVCL_WL08; OC-2 CVCL_WL09; OC-3 |
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