| Publication number |
CLPUB00494 |
|---|
| Authors |
Cavalloro R., Pozzi G., Zaffaroni G. |
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| Title |
Radiosensitivity and DNA content of an in vitro cell line of the Mediterranean fruit-fly. |
|---|
| Citation |
(In) Invertebrate systems in vitro; Kurstak E., Maramorosch K., Dubendorfer A. (eds.); pp.79-86; Elsevier; Oxford (1980) |
|---|
| Abstract |
Up to now, research on the sensitivity to ionising radiations of cell
lines of eukariotes in vitro has been carried out principally on plant
cells (Howland and Hart 1977) and on vertebrate animal cells (Puck 1960;
Watch and Okada 1969; Okumura and Uchiyama 1974), but not on invertebrate
cell lines. Stabilized cultures of cells of the Mediterranean fruit-fly
(Ceratitis capitate, Wied.), recently obtained in our laboratories
(Cavalloro, in press) have permitted the testing of such materials (Fig.
I, left part). The principal aim of the research was the ascertaining of
the radiosensitivity in relation to the DNA content of the nuclei of C.
capitata, and comparison with a line of Aedes albopictus (Skuse) (Singh
1967), which has a chromosome number half that of the Mediterranean fruit
fly (Southern 1976). The comparison between the two species was made with
both untreated material and material irradiated with gamma radiation,
using in parallel cytophotometric measurements of the DNA content in the
nuclei and measuring the survival and cell multiplication.
|
|---|
| Cell lines |
CVCL_W343; 45 M CVCL_Z884; CEC CC 130 |
|---|
