| Publication number |
CLPUB00493 |
|---|
| Authors |
Eppler A., Adam G., Sander E. |
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| Title |
Nutritional requirements and growth of two cell-lines from the aphid parasite, Ephedrus plagiator under different culture conditions. |
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| Citation |
(In) Invertebrate systems in vitro; Kurstak E., Maramorosch K., Dubendorfer A. (eds.); pp.59-62; Elsevier; Oxford (1980) |
|---|
| Abstract |
For the investigation of virus-vector relationships on a cellular level,
cell lines of vectors are a useful tool (Black 1969). Aphids are the most
prominent group of insect vectors of plant viruses and cell lines of
aphids would be a useful tool. However, no aphid cell lines are as yet
available (Valenta 1977). While dealing with primary cultures of the
Damson hop aphid Phorodon humuli Schrk. Two cell lines of the insect
Ephedrus plagiator (Aphidiidae, Hymenoptera), a parasite of the aphid were
established in 1976. The cell lines were derived from larval tissue. Since
parasite and aphid embryos develop under comparable conditions, the new
cell lines were tried as a tool to optimize media and culture conditions
for aphid cells. Several media developed for aphid cell culture, an insect
cell line medium and an artificial medium were tested.
|
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| Cell lines |
CVCL_WK70; IBT-EP 1 CVCL_WK36; IBT-EP 2 |
|---|
