| Publication number |
CLPUB00490 |
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| Authors |
Ying Chen, Yan-Hong Yi, Hong-Jing Han, Heng Cui, Dan-Hua Shen, Li-Hui Wei, Hong-Yan Cheng, Xue Ye, Rui-Qiong Ma, Xiao-Hong Chang; |
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| Title |
Establishment of human endometriosis-derived immortalized eutopic endometrium stromal and epithelial cell lines. |
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| Citation |
Int. J. Clin. Exp. Med. 9:16450-16458(2016) |
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| Web pages |
https://www.ijcem.com/files/ijcem0027741.pdf |
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| Abstract |
Endometriosis (EM) is commonly seen in women at reproductive age and can
have a significant social and psychological impact. However, reliable,
simple in vitro models of endometriosis are not common. To establish human
EM-derived immortalized cell lines, we transfected cells from eutopic and
ectopic endometria of endometriosis patients with the plasmid PCD2SV40T.
The cells were then screened with G418, and monoclonal cells were isolated
by the limited dilution method. Subclones were evaluated by karyotype
analysis, immunocytochemistry, reverse transcription-polymerase chain
reaction, cell doubling time, and nude mice implantation. The established
immortalized eutopic stromal cell line was named hEM15A, and the ectopic
epithelial cell line was named hEM5B2. hEM15A cells exhibited the
characteristics of endometrium stromal cells, and no abnormalities in
chromosome structure or number were observed. hEM5B2 cells exhibited the
characteristics of epithelial cells, but had a polyploid karyotype. Both
of these cell lines could be passaged continuously. No tumourigenicity was
observed. hEM15A is the first immortalized EM-derived eutopic endometrium
stromal cell line, and hEM5B2 is an immortalized ectopic endometrium
epithelial cell line from Asian EM tissues. These cells could facilitate
studies on the aetiology, molecular mechanisms, and therapeutic models of
EM.
|
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| Cell lines |
CVCL_WJ05; hEM15A CVCL_WJ06; hEM5B2 |
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