| Publication number |
CLPUB00487 |
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| Authors |
Claudia Vennegoor, Afke Jonker, D. van Smeerdijk, A. van Es, Philip Rumke; |
|---|
| Title |
Specificity of a monkey antiserum for a melanoma cell line IPC-48. |
|---|
| Citation |
Prot. Biol. Fluids 25:731-734(1978) |
|---|
| Web pages |
https://books.google.ch/books?id=v4jIDAAAQBAJ&pg=PA731&lpg=PA731&dq=%22ipc-48%22&source=bl&ots=LLJhFyh0z_&sig=ACfU3U1MyEudxyShRwVNDeCjuFkQOoUZzQ&hl=en&sa=X&ved=2ahUKEwithMyTzKThAhWoy6YKHW-pCBQQ6AEwAXoECBQQAQ#v=onepage&q=%22ipc-48%22&f=false |
|---|
| Abstract |
Surface antigens of human melanoma cells have been studied using
heterologous antisera from monkeys (Macaca speciosa). An antiserum against
shortly cultured melanoma cells was cytotoxic for cells from different
melanomas, brain and embryonal fibroblasts, but not for normal or tumor
cells other than melanoma. A second antiserum against a cell line (IPC-48)
reacted with IPC-48 cells only. These results suggest a specific antigen
for the melanoma cell line IPC-48. The antiserum against IPC-48 was used
to compare two solubilization methods of membrane antigens. Antigens
solubilized in 2M KI appeared to be purer than antigens extracted with
hypotonic salt, the recovery of membrane antigens being comparably high in
both cases.
|
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| Cell lines |
CVCL_WG89; IPC-48 |
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