| Publication number |
CLPUB00482 |
|---|
| Authors |
Motonobu Satoh, Nobuaki Yanai, Touho Yoshida, Masao Takeuchi; |
|---|
| Title |
Establishment and characterization of a murine astroglial cell line. |
|---|
| Citation |
Res. Commun. Inst. Ferment. 19:6-14(1999) |
|---|
| Web pages |
https://www.ifo.or.jp/rc_pdf/19.pdf |
|---|
| Abstract |
A murine cell line, designated as KT-5, was established from a brain tumor
induced by the interplacental injection of N-nitrosomethyl urea. KT-5
cells grew in vitro with a doubling time of 17h. The karyotype of KT-5
cells was pseudotriploid with a modal chromosome number of 61. KT-5 was
tumorigenic when transplanted ether intraperitoneally or subcutaneously
into C3H/He mouse. Immunocytochemical analysis demonstrated that KT-5 was
immunopositive for glial fibrillary acidic protein (GFAP) and S100 protein
but not for Thy-1, myelin basic protein, or neurofilament, suggesting that
KT-5 cell derives from a murine astrocyte. Conditioned medium of KT-5
contained neurite-promoting activity for neurons and growth-promoting
activity for KT-5 cells themselves.
|
|---|
| Cell lines |
CVCL_5549; KT-5 |
|---|
