| Publication number |
CLPUB00475 |
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| Authors |
Yin C.-H., Li J.-Q., He Q., Liu S.-S., He L.-S. |
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| Title |
Mutations and reduced expression of p53 gene are involved in HPV- independent oncogenesis of cervical cancer. |
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| Citation |
Int. J. Clin. Exp. Pathol. 10:4356-4362(2017) |
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| Web pages |
https://www.ijcep.com/files/ijcep0036136.pdf |
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| Abstract |
Cervical cancer is one of the most prevalent malignancies in women, which
has human papillomavirus infection as the major risk factor. Here we
report the establishment of a new HPV-negative cervical cancer cell line
and characterization of genetic alterations relevant to the pathogenesis
of cervical cancer. A new cervical cancer cell line was established by
extensive culture and natural selection of cells a cervical cancer sample
of a 53-year woman. Human papillomavirus was detected by ELISA and PCR.
The expression levels of p53 and k-ras were analyzed by immunocytochemical
staining, the mutations of p53 and k-ras were assessed by PCR-sequencing,
the sensitivity to cisplatin and taxol was measured by MTT assay,
chromosomal anomaly was detected by karyotyping. The newly established
cervical cancer cell line (CTCC-1) did not have detectable HPV DNA or HPV
viral particle. CTCC-1 cells had massive chromosomal changes and harbored
heterozygous mutations at 870 C>T (protein Pro223Leu) and 1022 G>T
(protein Val274Phe) of p53 gene (NM_000546) with reduced p53 expression
compared to C33A cells whereas k-ras was not mutated nor inhibited in CTCC-1
cells. CTCC-1 cells were more sensitive to cisplatin and taxol than Hela
but more resistant to taxol than C33A cells. Overexpressing wild type p53
significantly increased the sensitivity of CTCC-1 to those chemotherapy
agents. Mutant p53 might be responsible for the oncogenesis of some HPV-
negative cervical cancers.
|
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| Cell lines |
CVCL_VT59; CTCC-1 |
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