| Abstract |
An improved understanding of the feline retroviruses, FeLV and RD-114, and
endogenous sarc genes in relation to FOCMA and neoplastic transformation
depends mainly upon the availability of well characterized feline cell
cultures. Over the past decade we have attempted to establish tumor cell
lines from numerous spontaneous tumors of domestic house cats.
Contamination with foamy and other indigenous viruses destroyed many of
these cultures but we were successful in deriving several long-term
epithelial tumor cell lines and many embryo fibroblastic cell cultures of
both long and short duration. These cultures were treated extensively with
chemicals and/or infected with heterolo-gous retroviruses in an effort to
transform them, to induce endogenous viruses or to rescue endogenous sarc
genes. Of special interest was one embryo cell culture (CF-927) that has
been maintained in our laboratory for over nine years. This culture
underwent spontaneous transformation at late subpassage but transformed
sooner if treated with a chemical carcinogen or infected with FeLV,
subgroup C. In this paper we describe this particular embryo culture and
summarize major findings concerning expression of retrovirus, FOCMA and
endogenous sarc genes in the cultured cat cells.
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