| Publication number |
CLPUB00441 |
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| Authors |
Zupi G., Mauro F., Balduzzi M.A., Pardini M.C., Cavaliere R., Greco C. |
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| Title |
Established melanoma cell lines from different metastatic nodules of a single patient. A useful model for cancer therapy. |
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| Citation |
Proc. Am. Assoc. Cancer Res. 26:22.86-22.86(1985) |
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| Abstract |
The failure of some therapeutic trials as far as the occurrence of newly
developed or intrinsic drug resistance is concerned, has reinforced the
concept of tumor cell heterogeneity. However, investigations in this field
are still limited by the lack of suitable experimental model systems. Five
human melanoma cell lines (JRs) were derived from separate metastatic foci
of a young woman and established in our own laboratory. The main
biological features of JRs lines were characterized either in vitro (in
monolayer cultures, multicellular spheroids) or in vivo (xenografts in
nude mice). In addition, the response of the lines exhibiting an
acceptable plating efficiency in some drugs commonly used in melanoma
treatment, was also investigated and evaluated by means of cell cloning
assays. The JRs lines behave heterogeneously in their in vitro growth,
tumorigenicity in nude mice, clonogenicity as well as in their sensitivity
to cis-Diamminedichloroplatinum (II) and Melphalan. On the contrary, these
lines have shown a similarly high sensitivity to m-AMSA and 4-epi-
doxorubicin. These results demonstrate the biological and functional
heterogeneity existing among multiple melanoma metastases of a single
patient. Moreover, they suggest that JRs lines may serve as a useful model
system to speculate on implications of heterogeneity to melanoma biology
and consequences to anticancer therapy.
|
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| Cell lines |
CVCL_L880; JR1 CVCL_5780; JR8 |
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