| Publication number |
CLPUB00438 |
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| Authors |
Wan Mohd Tajuddin Wan Nur Baitty, T. Hwa Li, Nasuha Noor Azam, Sothilingan Selvalingam, Rozita Rosli, Nik Hassan Nik Norliza; |
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| Title |
Establishment and characterization of USM-BC-1 cell line derived from human urinary bladder cancer. |
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| Citation |
Int. J. Med. Sci. Biotechnol. 1:4-23(2013) |
|---|
| Web pages |
https://www.oiirj.org/ijmsb/aug2013/01.pdf |
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| Abstract |
Transitional cell carcinoma of the urinary bladder is one of the common
diseases that causes morbidity and mortality worldwide. In Malaysia,
bladder cancer is in the 10 most frequent cancer occurred among males.
Since most of the current urinary bladder cancer cell lines were
established from populations in western countries while significant
genotypic variations have been reported among various populations, this
project aimed at establishing the first continuous cell line for providing
an ideal cancer cell model for Malaysian population and nearby countries
acquires significance. The urinary bladder cancer cells were isolated from
bladder cancer tissues obtained from a Malay patient. The obtained cells
were cultured continuously over 30 passages. This cell line was
characterized by observing its morphology, growth characteristics,
presence of cytokeratin 7 and desmin markers, expression of Ki-67, VEGF as
well as p21 proteins. The authenticity of the obtained cells was
investigated using Short Tandem Repeat (STR) analysis and contamination by
Mycoplasma was ascertained. Results revealed that this newly established
cell line (USM-BC-1) has been well characterized as an ideal cancer cell
model which may prove useful in cancer research towards developing future
diagnostic and therapeutic targets, taking into consideration the
genotypic variations among Asian population.
|
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| Cell lines |
CVCL_VP41; USM-BC-1 |
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