| Publication number |
CLPUB00419 |
|---|
| Authors |
Jin-Woo Kim, Seok-Nyeon Bae, Sung-Eun Namkoong, Seung-Gyu Song, Heon-Hyeong Lee, Seung Jo Kim; |
|---|
| Title |
Establishment and characterization of new cancer cell line (CUMC-2) derived from human epithelial carcinoma of ovary. |
|---|
| Citation |
(In conference) Proceedings of the Korean obstetrics and gynecology conference; pp.53-53; Korean Society of Obstetrics and Gynecology; Seoul; South Korea (1989) |
|---|
| Web pages |
http://kiss.kstudy.com/thesis/thesis-view.asp?key=2428826 |
|---|
| Abstract |
We have established a new cancer cell line of human ovarian carcinoma in
long term culture. This cell line was obtained from 60 year-old patient
during initial operation without prior radiotherapy or chemotherapy. Cells
from tumor mass were grown in culture as monolayers with the separate use
of RPMI-1640 containing fetal cord serum. The growth curve of cell line
was measured comparably. The cell line has been maintained for one year,
and passaged over 80 times. The histologic pattern of tissue culture cell
line was indistinguishable from that of original ovarian carcinoma.
Heterotransplantation of tumor mass. Ultrastructural analysis of tumor
cell showed specific morphological feature and receptor study showed
negative for both estrogen and progesteron receptor. They also showed
human chromosome pattern, and showed specific ectopic hormone production.
This new cell line will be of utility in investigations of tumor specific
antigens, ectopic hormone production, immunotherapy, inununodiagnosis
and sensitivity to radiation and chemotherapeutic agents.
|
|---|
| Cell lines |
CVCL_RV35; CUMC-2 |
|---|
