| Authors |
Akiko Higashitani, Shinkichi Yokoyama, Yukitoshi Shimizu, Michihiko Katsuura, Kaori Akiba, Tetsuo Mitui, Mikiya Endo, Toshinuki Takano, Tadashi Hayashi; |
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| Abstract |
A new human leukemic cell line MG-S was established from the peripheral
blood of a patient of Down's syndrome with acute leukemia. MG-S cells
which grew in single cell suspension were blastic microscopically.
Karyotype of MG-S was 46,XY,-4,-12,-17,-17,+der(17),t(4;17)
(4qter->4q21::17p11->17qter),+21,+mar,+mar,7p+,7q+, 13p+. Cytochemically,
they were positive for acid phosphatase and periodic acid-Sciff reaction,
but negative for myeloperoxidase, alfa naphthyl butylate esterase, and
naphthol AS-D chloroacetate esterase. Electromicroscopically, 90% cells
demonstrated the reaction of platelet peroxidase at the nuclear envelope
and endplasmic reticulum. None of them showed the myeloperoxidase reaction.
They did not have demarcation membrane systems and alpha-granules Under
the surface marker analysis cells were positive for CD33, CD34, HLA-DR,
CD41a, but negative for CD42b, GlyA. From these findings, MG-S cells were
determined to be a megakaryoblastic leukemia cell line. By TPA treatment,
the cell growth was reduced to 83% of control, and cells were
differentiated along with megakaryocyte lineage but not with any other
erythrocyte. myelomonocyte, nor lymphocyte lineage. The cell growth
increased to 130% by GM-CSF but was not changed by IL-3, IL-6, or G-CSF.
No differentiation was obtained by the stimulation of IL-3, IL-6, G-CSF or
GM-CSF treatment. MG-S cells were shown to be useful for the study of
megakaryocyte differentiation, especially of the early stage.
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