| Publication number |
CLPUB00406 |
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| Authors |
Curtin N.J., Ince P.G., Gaunt K.L., Fowler M.J.F., James O.F.W., Bassendine M.F. |
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| Title |
Establishment of a cell line (PLC/NUT/1) containing integrated hepatitis B virus (HBV) DNA from a hormone associated human primary liver cell cancer. |
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| Citation |
Gut 25:A541-A541(1984) |
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| Web pages |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1432462/
|
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| Abstract |
A continuous human cell line has been established from the primary
hepatocellular carcinoma (PLC) of a British woman. The patient had no
serological markers of HBV infection (HBsAg.HBcAb, and HBsAb ELISA-Abott
all-ve X2). She had received long term sex hormone treatment for
endometriosis. The tumour was alphafoetoprotein (AFP) secreting and arose
in a non-cirrhotic liver. The cell line, designated PLC/NUT/1 grows as an
adhering monolayer; the cells are of epithelial type and secrete
alphafoetoprotein. Karyotyping has shown a chromosome mode of 63; 71%
falling within the range 51-67. There are eight identifiable chromosomal
markers confirming a clonal origin. Despite negative serum markers, HBV
DNA was shown to be integrated into the cellular DNA of PLC/NUT/1 by
nucleic acid hybridisation using a cloned HBV(32P) DNA probe. Digestion
with a restriction endonuclease enzyme. Hind III, which does not cut HBV
DNA. yielded only one major and two minor fragments (larger than hepatitis
B virion DNA) containing HBV DNA sequences. This suggests that there are
at most only three integration sites of HBV DNA in these cells unlike the
PLC/PRF/5 (Alexander) cell line in which there are 8+. When PLC/NUT/1 is
heterotransplanted subcutaneously into male athymic mice tumours develop
at the site of inoculation. These are primary liver cancer similar in
morphology to the original tumour and immunoperoxidase staining is
positive for HBsAg and AFP. These results suggest involvement of HBV in
this hormone associated PLC. The paucity of sites of integration of HBV
DNA into the DNA of this human PLC cell line will facilitate study of the
oncogenicity of HBV.
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| Cell lines |
CVCL_LJ92; PLC/NUT/1
|
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