| Abstract |
A Duroc fibroblast cell line has been established and the genetic
resources have been long-term stored. We obtained the cell line by primary
culture, subculture and cryopreservation, cells' viability were assessed,
microbial or other organisms contamination were excluded, isoenzyme
analysis and chromosome analysis were detected, three exogenous genes were
transfect into the cells, results showed the freezing has little influence
to the cell viability, the purity and the hereditary stability of the cell
line were in good condition, which could provide abundant genetic
materials for other researches in biological field.
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