| Publication number |
CLPUB00398 |
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| Authors |
Hayakawa O., Koizumi M., Yamauchi O., Yamamoto H., Kudo R., Hashimoto M. |
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| Title |
Establishment of a cell line derived from human uterine cervical adenocarcinoma and the sensitivity to anticancer drugs. |
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| Citation |
Nihon Sanka Fujinka Gakkai Zasshi 39:1489.2-1489.2(1987) |
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| Web pages |
https://ci.nii.ac.jp/naid/110002123498/en |
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| Abstract |
A new cell line of a human uterine cervical adenocarcinoma, designated as
CAC-1, was established from a moderately differenciated adenocarcinoma of
the cervix. CAC-1 was subcultivated more than 60 times during 14 months.
The population doubling time was 31 hours. Number of chromosome in the
majority of cells was hypertriploidy with a mode of 75 at the 51th passage.
In the cytoplasm, PAS, Alcian Blue and CEA stain-ing positive substance
could be seen. CA-125 and TPA was found in the cultured media. The
histology of the nude mice inoculated of the CAC-1 showed moderately
differentiated adenocarcinoma. And these histological findings closely
resembled to the original tumor. CAC-1 was also used for a drug
sensitivity test against 12 anticancer drugs by microcolonies inhibi-tion
test. The in vitro sensitivity was defined as less than 30% colony
survival. VP-16 and MMC showed sensitivity at peak plasma concentration
for 1 hour exposure. ACM, ACD, MMC, VP-16, ADM, VCR, VLB and CDDP showed
sensitivity at 1/10 peak plasma concentration for 24 hours exposure.
|
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| Cell lines |
CVCL_JX14; CAC-1 [Human cervical adenocarcinoma] |
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