| Abstract |
A new cell line, designated as TMCC-1, has been established from the
pleural effusion of a patient with uterine cervical adenocarcinoma. The
primary culture was performed in January, 1986. The cell line TMCC-1 was
subcultured serially in RPMI 1640 with 10% FCS more than 46 times in
March, 1987. The monolayer culture cells showed a pavement-like
arrangement with polygonal cells and had a tendency to pile up without
contact inhibition. PAS and Alcian Blue positive substances could be seen
in the cytoplasm. Desmosome structure, microvilli and secretory granules
were found by electron microscopy. The cell doubling time, saturation
density and plating efficiency were 53 hours, 2.9 X 105/cm2 and 16%,
respectively. Chromosomes analysis revealed hypotriploidy with modal
number of 65. No apparent marker chromosome was present. The nuclear DNA
histogram showed two peaks at 2.8 C and 5.6 C. The TMCC-1 cells were
transplanted subcutaneously into nude mice which developed a low-
differenciated adenocarcinoma resembling the original tumor, and
sialomucin was found in the cytoplasm by High Iron Diamine-Alcian Blue
staining. Tumor marker assay of the cultured media revealed significantly
high values of CA125, TPA, SCC and ferritin compared with control media.
By a immuno-histochemical method, CA125 and CEA were demonstrated in the
cultured cells and transplanted tumor.
|
|---|
