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Cellosaurus publication CLPUB00394

Publication number CLPUB00394
Authors Todorova K., Ivanov I., Georgieva A., Lazarova S., Milcheva R., Dimitrov P., Dimitrov R., Russev R.
Title Fumonisin B1 cytotoxicity and subcellular localization in duck embryo cell line DEC 99.
Citation C. R. Acad. Bulg. Sci. 68:617-622(2015)
Web pages https://www.researchgate.net/publication/281775443
Abstract A comparative study on the cytotoxic effect of fumonisin B1 (FB1) was carried out on BALB/c 3T3 and DEC 99 cell lines. The newly tested cell line DEC 99 appeared as more sensitive than BALB/c 3T3 line according to the performed Neutral red uptake assay. Light microscopic investigations of DEC99 cultures treated with FB1 showed altered monolayer with free of cells spaces and abundance of dead cells. The immunocytochemical techniques (immunofluorescent and immunogold labelling) proved the influx of the toxin through the cell membranes. The toxin was visualized in the cytoplasm and in the nucleus of the treated cells.
Cell lines CVCL_JF07; DEC-99