| Abstract |
In spite of the establishment of modern techniques like PCR for virus
detection, virus isolations in susceptible fish cell lines are still the
basis for international certification of most notifiable viral fish
diseases mentioned by the OIE and the European Union. Cell cultures may
change in susceptibility. If no regular susceptibility checks are done in
the laboratory, using an alternative sensitive cell culture might
therefore increase the chance of isolating a certain virus. The CCB
(common carp brain) cell line, developed by and cultured according to
Neukirch et al., 1999, was originally developed for the diagnosis of koi
herpesvirus (KHV). Moreover, we isolated other viruses from diseased koi
in CCB cells. Besides KHV, myxoviruses, birna-and/or reoviruses (not all
isolates were identified) and rhabdoviruses (spring viraemia of carp
virus, SVCV) were obtained. The susceptibility of CCB cells was also
investigated for viruses isolated from golden ide, European eel and
salmonids. CCB cells and the standard cell lines used in our laboratory:
Epithelioma papulosum cyprinid, EPC, Chinook Salmon Embryo, CHSE and Eel
kidney, EK-1 for the different viruses were inoculated with the following
viruses: infectious pancreatic necrosis virus (IPNV, strains Sp, Ab and
Ni), viral haemorrhagic septicaemia virus (VHSV, serotype I, II and III),
infectious haematopoietic necrosis virus (IHNV), golden ide reovirus
(GiRV), chum salmon reovirus (CSV), herpesvirus anguillae (HVA), and
another eel virus designated A1B virus. Non-infected cell lines were
incubated as negative controls. Two passages were carried out in the cell
lines before quantification of virus infectivity by titration of
supernatants in 10-fold dilution steps. Table 1 summarizes the inoculated
viruses used, and results concerning titration of virus infectivity
obtained in both the routine cell lines and the CCB cell line.
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