Cellosaurus logo
expasy logo

Cellosaurus publication CLPUB00372

Publication number CLPUB00372
Authors Inoue H.
Title Establishment of insect continuous cell lines and its utilization for virus multiplication in vitro.
Citation JARQ 23:31-36(1989)
Web pages https://www.jircas.go.jp/en/publication/jarq/23/1/31
Abstract Cell culture of insects is one of the most important techniques for the basic research of life science and biotechnology. Since Grace first established the insect continuous cell line, one fourth century has passed. During this period, many culture media were developed and more than 51 cell lines of Lepidoptera were established; thus the cell culture became a mature technique in the field of establishment of continuous cell lines. The application of insect cell culture is expected for the virus production in vitro aiming at the pest control, and for the production of useful proteins by the Baculovirus cell line system. For the economic production of viruses or useful proteins, several problems, such as 1) establishment of highly proliferative cell line, 2) selection of cell highly susceptible to viruses, 3) development of low-cost medium, and 4) large-scale culture of cells, must be solved. In the present paper, I report the establishment of continuous cell lines of the silkworm, Bombyx mori, and its usefulness for the research on the relationship between the multiplication of Bombyx mori nuclear polyhedrosis virus and the culture condition of cells. In addition, the cell line of the cabbage armyworm, Mamestra brassicae, was also established.
Cell lines CVCL_Z108; SES-BoMo-15A
CVCL_Z127; SES-MaBr-1
CVCL_Z128; SES-MaBr-2
CVCL_Z129; SES-MaBr-3
CVCL_Z130; SES-MaBr-4
CVCL_Z131; SES-MaBr-5