| Publication number |
CLPUB00359 |
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| Authors |
Takuji Katayama, Mikio Danbara, Takumi Aoki, Sosei Okina, Takashi Ishida, Koji Eshima, Koji Miyazaki, Masaaki Higashihara; |
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| Title |
Establishment and characterization of a novel cell line (HATAK) derived from natural killer cell neoplasm corresponding to the myeloid/NK progenitor. |
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| Citation |
Kitasato Med. J. 43:38-48(2013) |
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| Web pages |
https://mlib.kitasato-u.ac.jp/homepage/ktms/kaishi/pdf/KMJ43-1/KMJ43-1p038-048.pdf |
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| Abstract |
Objective: Immature natural killer (NK) cell neoplasms are extremely rare.
We established a novel cell line named HATAK from a case of NK cell
lymphoblastic leukaemia/lymphoma and describe here the detailed
characteristics of HATAK.
Methods: Cells were cultured in standard medium. After establishing this
new cell line, we performed immunophenotyping of HATAK cells by flow
cytometry-based genotyping by reverse transcription polymerase chain
reaction (RT-PCR), and cytotoxicity assay by the 51Cr releasing method
before and after interleukin (IL)-2 and IL-15 stimulation. We also
investigated the activation status of lymphocyte function-associated
antigen-1 (LFA-1) on chemokine stimulation.
Results: The immunophenotype was positive for CD7, CD13, CD33, and CD56,
and negative for other T-and B-cell antigens. The T-cell receptor gene was
in a germline configuration. Transcripts of the molecules characteristic
for NK cells were detected by RT-PCR. Even under cytokine stimulation,
HATAK cells exhibited neither cytotoxic activity nor IFN (interferon)-
gamma production. Notably, LFA-1 had already been activated in a steady
state, and the stimulation from the fractalkine-CX3CR1 axis never exerted
pressure on its activation status.
Conclusions: HATAK corresponded to the myeloid/NK progenitor based on
normal NK cell ontogeny. This cell line might be useful to elucidate the
biological characteristics of normal NK cells as well as the pathogenesis
of NK neoplasms.
|
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| Cell lines |
CVCL_HA65; HATAK |
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