| Publication number |
CLPUB00348 |
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| Authors |
Rangel-Corona R., Ramirez-Gaytan J.L., Rocha-Zavaleta L., Solorza-Luna G., Monroy-Garcia A., Martinez-Perez R.D., Trejo-Becerril C., de Jesus Ibarra-Sanchez M., Weiss-Steider B. |
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| Title |
Establishment and characterization of a cell line (CaLo) and a clone (KaLo), obtained from a cervix carcinoma and the effect of IL-2, IL-3, GM-CSF, M-CSF, TNF-alpha and IFN-gamma on its proliferation. |
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| Citation |
Rev. Inst. Nac. Cancerol. (Mex.) 39:1861-1866(1993) |
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| Web pages |
https://www.researchgate.net/publication/287882353https://pesquisa.bvsalud.org/ses/resource/pt/lil-135090 |
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| Abstract |
This work describes the morphological, chromosomal and presence of
desmosomes of a cell line (CaLo) derived from a biopsy of cervical cancer.
Our results indicate that CaLo has a typical morphology of epithelial
cells, a chromosomal aneuploidy with modal number of 58 and the presence
of desmosomes of bead to membrane positivity for the presence of
desmoglein-1. From CaLo a clone called KaLo was also isolated. This
cloning gives us evidence of the malignant origin of these cells. Both
cell types were cultured in the presence of some cytokines recently used
in certain clinical protocols (TNF-alpha, IL-2, IL-3 GM-CSF, M-CSF and
IFN-gamma). Our results demonstrate an inhibitory effect of TNF-alpha, IL-3
and GM-CSF proliferation, whereas with M-CSF and IFN-gamma, no effect was
detected. On the other hand, we observed an increase in cell proliferation
in the presence of IL-2. These results suggest that TNF-alpha, IL-3 and
M-CSF may have clinical applicability because of their inhibitory properties;
While highlighting the risk of using in vivo IL-2, as it activates the
proliferation of tumor cells of the cervix, as has been reported for some
head and neck carcinomas.
|
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| Cell lines |
CVCL_5121; CaLo |
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