| Publication number |
CLPUB00345 |
|---|
| Authors |
Kayajima T., Shirahama T., Yanase I., Ohi Y. |
|---|
| Title |
Characterization of a new cell line established from a human urinary-bladder cancer, with special reference to metastatic ability to the lung. |
|---|
| Citation |
Hinyoki Geka 2:577-580(1989) |
|---|
| Abstract |
Tissue cultivation of human cancer-deficient cancer cells was performed to
establish a human cancer-derived cultured cell line (BOY) that causes high
rate lung metastasis by subcutaneous transplantation of nude mice.
Cultured cells harvested stable growth from the early stage and reached 62
passages in 14 months. Cell doubling time was from 14.4 hours to 25.6
hours, and the colony formation rate was 54.3% to 64.0%. The number of
chromosomes ranged from 28 to 77 and a peak was observed around 62. Tumor
cancer was formed in 11 of 13 rats (84.6%) by cortical transplantation of
nude mice, and histologically, a histological image similar to the primary
tumor demyelitis was observed. Lung metastases were also found in 9 of 11
rats (81.8%) who developed subcutaneous tumors.
|
|---|
| Cell lines |
CVCL_7948; BOY-12E |
|---|
