| Abstract |
A new human pancreatic cancer cell line, designated YPK-1, was established
from ascites collected from a 62-year-old Japanese man. This cell line has
been maintained in vitro for 96 months with stable growth through 260
passages. We characterized the YPK-1 cell line morphology, doubling time,
levels of tumor markers secreted into culture medium, chromosomal content,
and tumorigenicity in nude mice. YPK-1 cells have a clear polygonal-shaped
cytoplasm, round nuclei, and prominent nucleoli. The cells grew as an
adherent monolayer and lacked contact inhibition. The doubling time at the
tenth passage was 49.3 hours. YPK-1 cells secreted detectable amounts of
carcinoembryonic antigen, carbohydrate antigen 19-9, carbohydrate antigen
125, carbohydrate antigen 50, DU-PAN-2, and Span-1. Chromosome analysis
revealed that the diploid chromosome number ranged from 57 to 62 with a
mode of 61. Tumorigenicity was demonstrated by development of tumors after
subcutaneous injection of YPK-1 cells into nude mice. We believe that this
cell line will contribute to studies of the biological and molecular
properties of pancreatic carcinoma and the development of therapeutic
strategies for this tumor.
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