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Cellosaurus publication CLPUB00332
| Publication number | CLPUB00332 |
|---|---|
| Authors | Xiu-Fang Wang, Zhan-Jun Lu, Fu-Ying Liu, Jie Hou, Jun-Xu Liu, Zeng-Nian Xu, Qiu-Yan Liu; |
| Title | Clonal cell selection and comparison study of S180 cell lines. |
| Citation | Zhongguo Shi Yan Dong Wu Xue Bao 2000:136-141(2000) |
| Web pages | https://oversea.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFD&dbname=CJFD2000&filename=ZGSD200003002&uniplatform=OVERSEA |
| Abstract | Objective: To isolate S180 stored in Beijing Cancer Institute and our department by the single cell cloning technique and to study property of those clonal cells. Methods: SDS polyacrylamide gel electrophoresis, scanning electron microscopy and immunohistochemical method were used. Results: Five sub-lines (S2H7, S1F11, S2D7, S2D9, S1B11) were isolated from S180 stored in Beijing Cancer Institute, three sub-lines (S1H10, S3F5, S1E11) were isolated from S180 stored in our department. Ascites of three sub-lines S1H10, S3F5 and S2H7 were bloody; S1F11 was tiny bloody; S1E11, S2D7, S2D9, S1B11 were not bloody property in 7 days of intraperitoneal inoculation. As for surficial structure under scanning electron microscope S3F5 had plentiful microvilli; S1B11 surface had a lot of prominences; S1F11 surficial microvilli were fewer than S3F5; S2D9 and S2H7 had different many tiny crests and tiny folds; S2D7 was more smooth. DNA contents were 8.5, 9.3, 7.9, 8.5, 8.6, 8.3 pg for six sublines S1B11, S2D7, S3F5, S2H7, S2D9, S1F11 respectively by flow cytometry. With immunohistochemistry the different clonal cells had different reactions with 11 kinds of antibodies (once reaction positive, positive rate was more than 97%). S1H10 and S2D9 were detected by SDS-PAGE, with 33 and 30 protein bands respectively. Conclusions: Eight sub-lines showed different properties. |
| Cell lines | CVCL_BS87; S180-S1B11 CVCL_IM39; S180-S1E11 CVCL_BS88; S180-S1F11 CVCL_BS89; S180-S1H10 CVCL_BS90; S180-S2D7 CVCL_BS91; S180-S2D9 CVCL_IM38; S180-S2H7 CVCL_IM37; S180-S3F5 |