| Publication number |
CLPUB00322 |
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| Authors |
Chen L.-Z., Huang J.-X., You L.-B., Li H.-F. |
|---|
| Title |
Establishment of human laryngeal alveolar rhabdomyosarcoma cell line (PLA-802) and observations on its biological properties. |
|---|
| Citation |
Jie Fang Jun Yi Xue Za Zhi 8:249-252(1983) |
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| Web pages |
https://en.cnki.com.cn/Article_en/CJFDTOTAL-JFJY198304003.htm |
|---|
| Abstract |
This cell line designated as PLA-802 was derived from the pleuro-pulmonary
metastatic tumour of a patient with laryngeal alveolar rhabdomyosarcoma 8
hrs after death. It had been transferred for 168 passages in a period of
twenty three months. The tumour cells of the cell line consisted of two
cytologic types, round and fusiform. Cross-striation was demonstrated in
the cells under light microscopy. On electron microscopic observation
parallel microfilaments with altered Z band had been found. The growth
curve of the cell line was determined from the 56th and 104th passages and
the cell number were counted daily for a week in each session. The growth
curves were similar in both passages. The doubling time of cell
proliferation was 42 hrs. The mode chromosome was 84 and the marker
chromosomes in most cells were present in the form of large telomere in
the D group. The characteristics of this cell line are rapid proliferation,
high malignancy and with properties of myogenic tumour. Other observations
such as the Con-A agglutination, planting efficiency, animal
transplantation and electron microscopic examination are briefly discussed.
|
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| Cell lines |
CVCL_AX34; PLA-802 |
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